Phospho mtor antibody

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August undefined, 2024

Web2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted … WebAn antibody to the phosphorylated S6 ribosomal protein was used to stain the tumors, all high-grade sarcomas. Pretreatment biopsy or resection material was tested: the original tumor (n=6) or tumor recurrence/metastasis (n=14); either of these may have been after treatment with other agents.

Phospho-mTOR (Ser2448) (E11) rabbit mAb FITC Conjugate

Web2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted 1:1000 (Cell Signaling Technology, Beverly, MA). β-actin antibody was diluted 1:5000 and FKBP12 antibodies were diluted 1:200 (Santa Cruz, Santa Cruz, CA). Anti-rabbit and ... WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. impute thesaurus

Activation mTOR Pathway for WB Biocompare Antibody Review

WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. WebRabbit recombinant monoclonal mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free. Validated in WB, IHC, Dot and tested in Mouse, Human. WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … lithium medication weight gain

mTOR (phospho-T2446) peptide from Biorbyt - biocompare.com

Western Blot Detection of Phosphorylation Events - Bio-Rad

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR426 (2)] to mTOR (phospho S2448) - BSA and Azide free. Suitable for: IHC-P, … WebSupplier Page from Biorbyt for mTOR (phospho-T2446) peptide. Product Specs; Company Info; Reviews; Product Specs; Item mTOR (phospho-T2446 ... This peptide was used as immunogen to produce orb338873 (mTOR (phospho-T2446) antibody)The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. Add to … impute systemWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. lithium medication what is it used for

"WebmTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, … " - Phospho mtor antibody

Phospho mtor antibody

Anti-mTOR (phospho S2448) antibody (ab1093) Abcam

Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and … WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody.

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WebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho S2448) Host species Rabbit Tested applications Suitable for: IHC-P, WB more details … WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find …

WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit Synonym (s): FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1 eCl@ss: 32160702 NACRES: NA.41 Pricing and availability is not currently available. Properties biological source rabbit Quality Level 100 antibody form affinity purified immunoglobulin WebSep 5, 2024 · p-mTOR Antibody (59.Ser 2448) is an IgG1 κ mouse monoclonal p-mTOR antibody that detects Ser 2448 phosphorylated mTOR of mouse, rat and human origin by …

WebUnc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. WebPhospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site …

Webtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ...

WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … impute softwareWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … impute time series in rWebDownload Zoom Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting. Anti-phospho-mTOR (Thr2164) Antibody MSDS … impute value in pythonWebImmunofluorescent analysis of Phospho-mTOR (Ser2481) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X … impute time series pythonWebMTOR (2475) Expression: P42345 MTOR_HUMAN: Expressed in numerous tissues, with highest levels in testis. Description: an atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. impute the dataWebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for … imputets packageWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. lithium medicine generic