Cy5.5 confocal
WebNational Center for Biotechnology Information WebAdd products for $400 to your cart and get free express shipping . This Cyanine5.5 labeling reagent is a dye azide for click chemistry, available as 10 mM solution in DMSO, or in solid form. The dye possesses far red / near infrared emission, which allows to use it for NIR live organism imaging. Cyanine5.5 can replace Cy5.5® and DyLight 680.
Cy5.5 confocal
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WebCy5 HYQ - The Cy5 HYQ filter combination for red-wavelength excitation is designed to provide high-energy excitation of the fluorophore Cy5, although the wide excitation range (yellow-orange to red) allows the set to be utilized with a variety of fluorochromes. WebTo be sure the nanoparticles migrated into the cells, the near-IR dye Cy5.5 was affixed to the lactoferrin-labeled nanoparticles that were incubated with C6 cells for 24 h. Confocal microscopy showed signal inside the cells, confirming uptake of the SPIONs.
WebZeiss LSM 710 Confocal Laser Scanning Microscope; Zeiss LSM 800 Confocal Laser Scanning Microscope; Zeiss LSM 880 Confocal Laser Scanning Microscope; Zeiss Lumar V12 Fluorescence Stereomicroscope; ... 5) Cy5: Chroma set 41024, Excitation 590-650 nm, Emission 665 nm and above (long pass filter). WebApr 3, 2024 · We next investigated the uptake of Cy5.5-AuNPs in fixed brains sections, sampling brains from mice sacrificed 30 days after ICM delivery of Cy5.5-AuNPs. Using confocal microscope, we acquired …
WebCy5.5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. WebLeft: Cy3 and Cy5 tagged DNA duplex (S1:S2) added to fixed/permeabilised cells and imaged using confocal microscopy. Images A/E represents the Cy3 channel; B/F the Cy5 channel; C/G the bright ...
Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while …
WebCy5.5-labeled Affibody molecule for near-infrared fluorescent optical imaging of epidermal growth factor receptor positive tumors. Affibody protein is an engineered protein scaffold … greenstar auto parts orlando floridaWebFeb 25, 2024 · Cy5 was synthesized in accordance with a previously reported procedure 24. A 100 μM solution of Cy5 in PBS (pH 7.4) was generated from a 50 mM … fnaf discordedkitty as chicaWebFluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera. Automated … fnaf disney 2WebFor in vivo optical imaging, Cy5.5-NGR2 exhibited rapid HT-1080 tumor targeting at 0.5 h postinjection (pi), and highest tumor-to-background contrast at 2 h pi. The CD13-specific … green star australia rating systemWebResults: In this way Cy5.5 is measurable at 488-nm and 633-nm excitation. Further, with the two different filters it is possible to distinguish Cy5 from Cy5.5 in the same detection … greenstar auto recyclersWebCy5.5 is a cyanine dye consisting of two quaternized heteroaromatic bases (A and A’) joined by a polymethine chain with five carbons (14), which binds directly to the nanoparticles. … greenstar auto recyclers orlando flWebCytoFLEX Cytometer uses the sample peristaltic pump to take sample and calibrates sample volume delivery for absolute cell counts without using beads. It can process … fnaf disney movie sister location